Home > Sandwich ELISA Kits > Human Protein-glutamine gamma-glutamyltransferase 4 (TGM4) ELISA Kit

Product Name:

Human Protein-glutamine gamma-glutamyltransferase 4 (TGM4) ELISA Kit

Cat.#:

JL17310

Regulatory Status:

RUO

Alternative:

TGP; hTGP; Fibrinoligase; Prostate-specific transglutaminase; Transglutaminase P;Transglutaminase 4, Prostate

Detection Method:

ELISA

Assay Type:

Sandwich

Detection Range:

0.781-50ng/mL

Sensitivity:

0.4ng/mL

Specificity:

This kit recognizes Human TGM4 in samples. No significant cross-reactivity or interference between Human TGM4 and analogues was observed.

Sample Type:

Serum, plasma, tissue homogenates and other biological fluids.

Assay Procedure:

Research Area:

Cell Biology

Size & Price:

Human A1BG Polyclonal Antibody

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Product Datasheets

Instruction:

Data
Citations
Sequence / 3D

| TGM4

Protein-glutamine gamma-glutamyltransferase 4 is an enzyme that is encoded by the TGM4 gene.Predicted to enable protein-glutamine gamma-glutamyltransferase activity. Predicted to be involved in peptide cross-linking. Predicted to act upstream of or within mating plug formation. Located in Golgi apparatus and collagen-containing extracellular matrix.

| Typical data

As the OD values of the standard curve may vary according to the conditions of the actual assay performance (e.g. operator, pipetting technique, wash technique or temperature effects), the operator should establish a standard curve for each test. Typical standard curve and data are provided below for reference only.

Concentration (ng/mL)	50	25	12.5	6.25	3.125	1.562	0.781	0
OD	2.29	1.29	0.71	0.43	0.29	0.2	0.18	0.07
Corrected OD	2.22	1.22	0.64	0.36	0.22	0.13	0.11	-

| Repeatability

Intra-assay Precision (Precision within an assay)：CV% < 10%; Inter-assay Precision (Precision between assays)：CV% < 10%

| Recovery

The recovery of Human TGM4 spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

Sample Type	Range(%)	Average Recovery(%)
Serum(n=8)	83-101	96
Plasma(n=8)	92-105	102
Cell Culture Supernates(n=8)	96-108	104

| Linearity

Samples were spiked with high concentrations of Human TGM4 and diluted with Universal Diluent to produce samples with values within the range of the assay.

		Serum(n=4)	Plasma(n=4)	Cell Culture Supernates(n=4)
1 : 2	Range (%)	84-95	85-96	90-110
1 : 2	Average (%)	91	93	96
1 : 4	Range (%)	89-103	87-108	105-115
1 : 4	Average (%)	94	98	109